La FIM ha dado a conocer la lista provisional de pilotos que tomarán parte en el Campeonato del Mundo de MotoGP™ de 2016.
| NÚMERO. | PILOTO | PAÍS | EQUIPO | MOTO |
| 4 | ANDREA DOVIZIOSO | ITALIA | DUCATI RACING TEAM | DUCATI |
| 6 | STEFAN BRADL | ALEMANIA | APRILIA RACING TEAM GRESINI | APRILIA |
| 8 | HÉCTOR BARBERÁ | ESPAÑA | AVINTIA RACING | DUCATI |
| 9 | DANILO PETRUCCI | ITALIA | OCTO PRAMAC RACING | DUCATI |
| 15 | ALEX DE ANGELIS | SAN MARINO | IODARACING PROJECT | ART |
| 19 | ÁLVARO BAUTISTA | ESPAÑA | APRILIA RACING TEAM GRESINI | APRILIA |
| 25 | MAVERICK VIÑALES | ESPAÑA | TEAM SUZUKI ECSTAR | SUZUKI |
| 26 | DANI PEDROSA | ESPAÑA | REPSOL HONDA TEAM | HONDA |
| 29 | ANDREA IANNONE | ITALIA | DUCATI RACING TEAM | DUCATI |
| 35 | CAL CRUTCHLOW | REINO UNIDO | LCR HONDA | HONDA |
| 38 | BRADLEY SMITH | REINO UNIDO | MONSTER YAMAHA TECH 3 | YAMAHA |
| 41 | ALEIX ESPARGARÓ | ESPAÑA | TEAM SUZUKI ECSTAR | SUZUKI |
| 43 | JACK MILLER | AUSTRALIA | MARC VDS RACING TEAM | HONDA |
| 44 | POL ESPARGARÓ | ESPAÑA | MONSTER YAMAHA TECH 3 | YAMAHA |
| 45 | SCOTT REDDING | REINO UNIDO | OCTO PRAMAC RACING | DUCATI |
| 46 | VALENTINO ROSSI | ITALIA | MOVISTAR YAMAHA MotoGP | YAMAHA |
| 50 | EUGENE LAVERTY | IRLANDA | ASPAR MotoGP TEAM | DUCATI |
| 53 | TITO RABAT | ESPAÑA | ESTRELLA GALICIA 0,0 MARC VDS | HONDA |
| 68 | YONNY HERNÁNDEZ | COLOMBIA | ASPAR MotoGP TEAM | DUCATI |
| 76 | LORIS BAZ | FRANCIA | AVINTIA RACING | DUCATI |
| 93 | MARC MÁRQUEZ | ESPAÑA | REPSOL HONDA TEAM | HONDA |
| 99 | JORGE LORENZO | ESPAÑA | MOVISTAR YAMAHA MotoGP | YAMAHA |