Marc Márquez doesn’t yield neither in the free practice session and He has been the fastest of the first practice session of the MotoGP Grand Prix of Czech Republic in a session which Lorenzo has dominate.
Results:
| Pos. | Nº | Rider | Team | Motorbike | Diff | Gap | Laps | |
| 1 | 93 | Marc MARQUEZ | SPA | Repsol Honda Team | Honda | 1:56.803 | 17 | |
| 2 | 99 | Jorge LORENZO | SPA | Movistar Yamaha MotoGP | Yamaha | + 00,037 | + 00,037 | 15 |
| 3 | 26 | Dani PEDROSA | SPA | Repsol Honda Team | Honda | + 00,015 | + 00,052 | 17 |
| 4 | 41 | Aleix ESPARGARO | SPA | NGM Forward Racing | Forward Yamaha | + 00,147 | + 00,199 | 15 |
| 5 | 46 | Valentino ROSSI | ITA | Movistar Yamaha MotoGP | Yamaha | + 00,063 | + 00,262 | 18 |
| 6 | 6 | Stefan BRADL | GER | LCR Honda MotoGP | Honda | + 00,019 | + 00,281 | 18 |
| 7 | 4 | Andrea DOVIZIOSO | ITA | Ducati Team | Ducati | + 00,085 | + 00,366 | 15 |
| 8 | 68 | Yonny HERNANDEZ | COL | Energy T.I. Pramac Racing | Ducati | + 00,021 | + 00,387 | 17 |
| 9 | 44 | Pol ESPARGARO | SPA | Monster Yamaha Tech 3 | Yamaha | + 00,444 | + 00,831 | 18 |
| 10 | 38 | Bradley SMITH | GBR | Monster Yamaha Tech 3 | Yamaha | + 00,182 | + 01,013 | 16 |
| 11 | 51 | Michele PIRRO | ITA | Ducati Team | Ducati | + 00,224 | + 01,237 | 14 |
| 12 | 29 | Andrea IANNONE | ITA | Pramac Racing | Ducati | + 00,021 | + 01,258 | 14 |
| 13 | 45 | Scott REDDING | GBR | GO&FUN Honda Gresini | Honda | + 00,240 | + 01,498 | 15 |
| 14 | 35 | Cal CRUTCHLOW | GBR | Ducati Team | Ducati | + 00,039 | + 01,537 | 16 |
| 15 | 19 | Alvaro BAUTISTA | SPA | GO&FUN Honda Gresini | Honda | + 00,358 | + 01,895 | 16 |
| 16 | 8 | Hector BARBERA | SPA | Avintia Racing | Avintia | + 00,016 | + 01,911 | 14 |
| 17 | 7 | Hiroshi AOYAMA | JPN | Drive M7 Aspar | Honda | + 00,181 | + 02,092 | 18 |
| 18 | 17 | Karel ABRAHAM | CZE | Cardion AB Motoracing | Honda | + 00,321 | + 02,413 | 15 |
| 19 | 2 | Leon CAMIER | GBR | Drive M7 Aspar | Honda | + 00,743 | + 03,156 | 18 |
| 20 | 15 | Alex DE ANGELIS | RSM | NGM Forward Racing | Forward Yamaha | + 00,281 | + 03,437 | 13 |
| 21 | 70 | Michael LAVERTY | GBR | Paul Bird Motorsport | PBM | + 00,105 | + 03,542 | 16 |
| 22 | 2 | Leon CAMIER | GBR | Drive M7 Aspar | Honda | + 00,100 | + 03,642 | 16 |
| 23 | 9 | Danilo PETRUCCI | ITA | Octo IodaRacing Team | ART | + 00,052 | + 03,694 | 14 |
| 24 | 23 | Broc PARKES | AUS | Paul Bird Motorsport | PBM | + 00,255 | + 03,949 | 16 |